![]() ![]() Hence, for obtaining more accurate results, the spectrum needs to be taken at a constant or particular temperature.The position of the band maximum does not shift much with a decrease in temperature.Fine absorption bands will be produced when absorption occurs at lower temperatures due to the smaller distribution of excited states.When the temperature is decreased, rotational and vibrational energy states of the molecules also get lowered.Rotational and vibrational energy states depend on the temperature.Simple thermal expansion of the solution can change the intensity of the absorption band.However, total absorption intensity is independent of the temperature.With the decrease in temperature, the position of the peak (absorption maximum) moves very little towards the longer wavelength side.With the decrease in temperature, the sharpness of absorption bands increases.The change in temperature of the sample plays a role in the spectrum. List of factors affecting UV-Vis Spectroscopyįor measurement of absorbance and studies, there are numerous factors, which impact the results in ultraviolet visible spectroscopy. This blog takes you to the study of variables, which affect the spectrum obtained from UV-Vis spectroscopy. Hence, it is essential to study the factors influencing UV-Vis spectroscopy, so that a clear picture will be obtained for careful measurement of the spectrum. Reproducibility is missing if any parameters like temperature, pH, concentration are changed. Sometimes, the spectrum obtained from the UV-Vis spectrophotometer need not be the same every time. The obtained light intensity versus wavelength is called the spectrum. It is used particularly in ultraviolet and visible regions. It is performed through measurement of the intensity of light, which passes through a sample with respect to the intensity of light through a reference. UV-Vis spectroscopy is a quantitative laboratory technique used for the measurement of the absorbance of light by a chemical compound. ![]()
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